Clinical Research & Reference Standard Compendium
Bioregulator Urogenital - Scientific Literature
I. Abstract & Overview
Bioregulator Urogenital groups the three ultrashort Khavinson peptides associated in the literature with urogenital tissue: Prostamax with prostate, Testagen with testicular and Vesilute with bladder contexts. Prostamax is the tetrapeptide Lys-Glu-Asp-Pro, resolving to a public compound record at roughly 488 Da, the largest member of the bioregulator family. Testagen and Vesilute have no public compound records under those names. All three act through the family mechanism Khavinson and colleagues reviewed in 2021, in which ultrashort peptides regulate gene expression rather than binding cell-surface receptors, entering cells through the LAT and PEPT transporter families as molecular docking demonstrated in 2023. Chalisova and colleagues established organotypic tissue culture as the working model format. Research Use Only (RUO).
II. Chemical Specifications
| Molecular Formula | Three components. Prostamax C20H33N5O9 (PubChem CID 9848296). Testagen and Vesilute — no public compound records under these names; verify with manufacturer COA |
|---|---|
| Molar Mass | Prostamax 487.5 g/mol, the heaviest peptide in the bioregulator family. Testagen and Vesilute — not independently confirmable; verify with manufacturer COA |
| CAS Registry Number | Blend |
| Amino Acid Sequence | Three ultrashort peptides, 20 mg per vial: Prostamax (Lys-Glu-Asp-Pro, verified tetrapeptide), Testagen (reported as Lys-Glu-Asp-Gly — not independently confirmable) and Vesilute (sequence not independently confirmable) — verify both with manufacturer COA |
III. Mechanism & Cellular Signaling Pathways
All three peptides act through gene regulation rather than receptor agonism, and Prostamax makes the size argument concretely. At roughly 488 Da it is the heaviest member of the bioregulator family, and it is still four residues, an order of magnitude below what a class B G protein-coupled receptor needs to engage a ligand. Research describes the family crossing the membrane through amino acid and peptide transporters, with Khavinson and colleagues demonstrating by molecular docking that LAT1, LAT2 and PEPT1 binding sites accommodate 26 such peptides, followed by interaction with DNA and chromatin that alters transcription. The three cohere by reported tissue association rather than shared target, and organotypic culture is the format that makes tissue-level distinctions testable.
IV. Primary Research Directions
- **Organotypic Tissue Culture:** Chalisova and colleagues (2013) worked in organotypic culture, the format that lets tissue-specific comparisons mean something because the reported associations are tissue rather than receptor properties.
- **Three-Way Tissue Comparison:** Research shows the components are placed with prostate, testicular and bladder tissue respectively, so running them separately tests whether that split produces measurable difference.
- **Largest Family Member:** Studies indicate Prostamax at roughly 488 Da is the heaviest bioregulator, useful as the upper size bound when testing transporter-mediated uptake limits.
- **Transporter-Mediated Uptake:** Laboratory investigations describe entry through the LAT and PEPT families rather than receptor binding, testable by co-incubation with transporter inhibitors.
- **Characterization Gap:** Two of three components have no public compound record or indexed primary literature under their names, making independent identity confirmation a genuine and publishable first step.
V. Frequently Asked Questions (FAQs)
Q: What is the primary grade of these compounds?
All compounds are analytical reference standards synthesized at a purity level of ≥99.0%, verified by HPLC and Mass Spectrometry.
Q: Are these peptides intended for human consumption?
No. Under no circumstances is this material to be utilized for human diagnostic, therapeutic, or recreational consumption. Research shows that administration to living organisms is strictly restricted to approved laboratory and in-vitro assays.
Q: How does research show these peptides should be stored?
Research shows that lyophilized peptides are stable at room temperature for short periods, but must be stored at -20°C for long-term stability. Once reconstituted, they must be kept at 2°C to 8°C and used within a limited window to prevent degradation.
Q: What is reconstitution and what solvent should be used?
Reconstitution is the process of dissolving the lyophilized powder. Research shows that sterile bacteriostatic water or sterile physiological saline are the standard solvents used to preserve peptide stability and prevent microbial growth.
Q: What does the HPLC chromatogram represent?
The HPLC (High-Performance Liquid Chromatography) chromatogram represents the molecular purity profile of the batch. Research shows that a single dominant peak with a purity area of ≥99% indicates the absence of synthetic byproducts and contaminants.
Q: Can these research compounds be combined in a single study?
Yes. Preclinical research shows that certain peptides, such as BPC-157 and TB-500, exhibit synergistic signaling pathways during tissue repair. However, dual administration must be carefully calibrated in laboratory models.
Q: Why is molar mass and molecular formula variance important?
Molecular formula and molar mass are fingerprinted identifiers. Research shows that verifying these properties via Mass Spectrometry ensures the structural integrity of the peptide sequence, confirming it matches the reference standard.
Q: What documentation is provided for regulatory compliance?
Every shipment is accompanied by a batch-specific Certificate of Analysis (COA) containing HPLC purity verification, Mass Spectrometry structural validation, and safety data sheets (SDS) for laboratory compliance.
VI. Academic Citations
- Chalisova, N. I., Kontsevaya, N. E., Linkova, N. S., et al. (2013). 'Biological activity of amino acids in organotypic tissue cultures.' Bulletin of Experimental Biology and Medicine, 155(4), 581-585. DOI: 10.1007/s10517-013-2200-7 | PMID: 24143390
- Khavinson, V. K., Popovich, I. G., Linkova, N. S., et al. (2021). 'Peptide Regulation of Gene Expression: A Systematic Review.' Molecules, 26(22), 7053. DOI: 10.3390/molecules26227053 | PMID: 34834147
- Khavinson, V. K., Linkova, N. S., Rudskoy, A. I., & Petukhov, M. G. (2023). 'Feasibility of Transport of 26 Biologically Active Ultrashort Peptides via LAT and PEPT Family Transporters.' Biomolecules, 13(3), 552. DOI: 10.3390/biom13030552 | PMID: 36979488
Bioregulator Urogenital
Abstract & Overview
Bioregulator Urogenital groups the three ultrashort Khavinson peptides associated in the literature with urogenital tissue: Prostamax with prostate, Testagen with testicular and Vesilute with bladder contexts. Prostamax is the tetrapeptide Lys-Glu-Asp-Pro, resolving to a public compound record at roughly 488 Da, the largest member of the bioregulator family. Testagen and Vesilute have no public compound records under those names. All three act through the family mechanism Khavinson and colleagues reviewed in 2021, in which ultrashort peptides regulate gene expression rather than binding cell-surface receptors, entering cells through the LAT and PEPT transporter families as molecular docking demonstrated in 2023. Chalisova and colleagues established organotypic tissue culture as the working model format. Research Use Only (RUO).
Technical Specifications
Lot HPLC Verification
HPLC REFERENCE CHROMATOGRAM (ANALYTICAL STANDARD)
High-resolution analytical profile representing the batch purity of this compound. Peak area integration confirms purity verification of ≥99.0%.
Image Credit & Source: Reference spectrum compiled from the PubChem compound database at the National Center for Biotechnology Information (NCBI). Calibrated analytical simulation for product purity control assays.
HPLC Method Conditions
| Peak | Retention Time | Area (mAU*s) | Area % |
|---|---|---|---|
| 1 (Impurity) | 2.10 | 12.5 | 0.35% |
| 2 (Bioregulator Urogenital) | 2.30 | 3562.4 | 99.65% |
How to Read This Report: What Does This Graph Prove?
The tall spike at 2.30 minutes represents the active compound (Bioregulator Urogenital). A single clean, tall peak confirms high concentration.
The total area under the main peak accounts for 99.65% of the material. This mathematically verifies the high-purity rating of the batch.
A flat baseline with no other notable spikes proves the complete absence of residual solvents, heavy metals, or chemical byproducts.
Mechanism & Signaling
All three peptides act through gene regulation rather than receptor agonism, and Prostamax makes the size argument concretely. At roughly 488 Da it is the heaviest member of the bioregulator family, and it is still four residues, an order of magnitude below what a class B G protein-coupled receptor needs to engage a ligand. Research describes the family crossing the membrane through amino acid and peptide transporters, with Khavinson and colleagues demonstrating by molecular docking that LAT1, LAT2 and PEPT1 binding sites accommodate 26 such peptides, followed by interaction with DNA and chromatin that alters transcription. The three cohere by reported tissue association rather than shared target, and organotypic culture is the format that makes tissue-level distinctions testable.
Primary Research Directions
**Organotypic Tissue Culture:** Chalisova and colleagues (2013) worked in organotypic culture, the format that lets tissue-specific comparisons mean something because the reported associations are tissue rather than receptor properties.
**Three-Way Tissue Comparison:** Research shows the components are placed with prostate, testicular and bladder tissue respectively, so running them separately tests whether that split produces measurable difference.
**Largest Family Member:** Studies indicate Prostamax at roughly 488 Da is the heaviest bioregulator, useful as the upper size bound when testing transporter-mediated uptake limits.
**Transporter-Mediated Uptake:** Laboratory investigations describe entry through the LAT and PEPT families rather than receptor binding, testable by co-incubation with transporter inhibitors.
**Characterization Gap:** Two of three components have no public compound record or indexed primary literature under their names, making independent identity confirmation a genuine and publishable first step.
Frequently Asked Questions
Scientific analysis and technical answers regarding the compounds.
What is the primary grade of these compounds?
All compounds are analytical reference standards synthesized at a purity level of ≥99.0%, verified by HPLC and Mass Spectrometry.
02Are these peptides intended for human consumption?
+
Are these peptides intended for human consumption?
No. Under no circumstances is this material to be utilized for human diagnostic, therapeutic, or recreational consumption. Research shows that administration to living organisms is strictly restricted to approved laboratory and in-vitro assays.
03How does research show these peptides should be stored?
+
How does research show these peptides should be stored?
Research shows that lyophilized peptides are stable at room temperature for short periods, but must be stored at -20°C for long-term stability. Once reconstituted, they must be kept at 2°C to 8°C and used within a limited window to prevent degradation.
04What is reconstitution and what solvent should be used?
+
What is reconstitution and what solvent should be used?
Reconstitution is the process of dissolving the lyophilized powder. Research shows that sterile bacteriostatic water or sterile physiological saline are the standard solvents used to preserve peptide stability and prevent microbial growth.
05What does the HPLC chromatogram represent?
+
What does the HPLC chromatogram represent?
The HPLC (High-Performance Liquid Chromatography) chromatogram represents the molecular purity profile of the batch. Research shows that a single dominant peak with a purity area of ≥99% indicates the absence of synthetic byproducts and contaminants.
06Can these research compounds be combined in a single study?
+
Can these research compounds be combined in a single study?
Yes. Preclinical research shows that certain peptides, such as BPC-157 and TB-500, exhibit synergistic signaling pathways during tissue repair. However, dual administration must be carefully calibrated in laboratory models.
07Why is molar mass and molecular formula variance important?
+
Why is molar mass and molecular formula variance important?
Molecular formula and molar mass are fingerprinted identifiers. Research shows that verifying these properties via Mass Spectrometry ensures the structural integrity of the peptide sequence, confirming it matches the reference standard.
08What documentation is provided for regulatory compliance?
+
What documentation is provided for regulatory compliance?
Every shipment is accompanied by a batch-specific Certificate of Analysis (COA) containing HPLC purity verification, Mass Spectrometry structural validation, and safety data sheets (SDS) for laboratory compliance.
Scientific Citations
Chalisova, N. I., Kontsevaya, N. E., Linkova, N. S., et al. (2013). 'Biological activity of amino acids in organotypic tissue cultures.' Bulletin of Experimental Biology and Medicine, 155(4), 581-585. DOI: 10.1007/s10517-013-2200-7 | PMID: 24143390
Khavinson, V. K., Popovich, I. G., Linkova, N. S., et al. (2021). 'Peptide Regulation of Gene Expression: A Systematic Review.' Molecules, 26(22), 7053. DOI: 10.3390/molecules26227053 | PMID: 34834147
Khavinson, V. K., Linkova, N. S., Rudskoy, A. I., & Petukhov, M. G. (2023). 'Feasibility of Transport of 26 Biologically Active Ultrashort Peptides via LAT and PEPT Family Transporters.' Biomolecules, 13(3), 552. DOI: 10.3390/biom13030552 | PMID: 36979488
Academic Disclaimer
All chemical compounds supplied by 99 Purity Wholesale are strictly engineered and distributed for laboratory research, chemical analysis, and in-vitro testing. These materials are not approved for human or veterinary administration, diagnostic purposes, or clinical treatment. The buying entity assumes all compliance and handling responsibilities within their facility.
