Clinical Research & Reference Standard Compendium
Melanocortin & Reproductive - Scientific Literature
I. Abstract & Overview
Melanocortin & Reproductive supplies Melanotan-II (10 mg), PT-141 (10 mg) and Kisspeptin-10 (10 mg) as three separately labelled lyophilized vials. Two are lactam-bridged cyclic melanocortin agonists separated by approximately one dalton — PT-141 is the deamidated form of Melanotan-II — making this the panel with the most demanding identity verification requirement in the range. The third acts at KISS1R, a receptor upstream of and unrelated to the melanocortin family. Supplied for Research Use Only.
II. Chemical Specifications
| Molecular Formula | Blend — no single formula applies; Melanotan-II C50H69N15O9, PT-141 C50H68N14O10, Kisspeptin-10 C63H83N17O14 |
|---|---|
| Molar Mass | Melanotan-II 1024.2 g/mol; PT-141 1025.2 g/mol — approximately one dalton apart; Kisspeptin-10 1302.4 g/mol |
| CAS Registry Number | Blend |
| Amino Acid Sequence | Melanotan-II Ac-Nle-cyclo(Asp-His-D-Phe-Arg-Trp-Lys)-NH2; PT-141 the same cyclic scaffold with a free acid terminus; Kisspeptin-10 Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2 |
III. Mechanism & Cellular Signaling Pathways
Melanotan-II and PT-141 are cyclic analogs of alpha-melanocyte stimulating hormone closed by a lactam bridge, acting across melanocortin receptor subtypes; the difference between them is a carboxy-terminal amide against a free acid, worth roughly one dalton. Melanotan-II is broadly active rather than subtype selective, a limitation the 2024 tool compound guidance from Weirath and Haskell-Luevano addresses directly. Kisspeptin-10 acts at KISS1R, identified by Kotani and colleagues in 2001 as the receptor for the KiSS-1 gene product, and it operates at the hypothalamic level upstream of gonadotropin release.
IV. Primary Research Directions
- High-resolution observed mass printed for both melanocortin vials, the only way to distinguish two compounds separated by approximately one dalton
- Two lactam-bridged cyclic peptides alongside one linear decapeptide, giving directly comparable cyclic and linear stability behaviour within a single tray
- A hypothalamic KISS1R agonist supplied alongside downstream melanocortin agonists, allowing an observation to be localised by receptor family rather than assumed
- Written statement that Melanotan-II is a broad rather than subtype-selective agonist, with reference to the 2024 melanocortin tool compound guidance
- Handling protocol advising the two near-isobaric vials are never open simultaneously, since a mislabelled aliquot cannot be recovered by routine assay
V. Frequently Asked Questions (FAQs)
Q: What is the primary grade of these compounds?
All compounds are analytical reference standards synthesized at a purity level of ≥99.0%, verified by HPLC and Mass Spectrometry.
Q: Are these peptides intended for human consumption?
No. Under no circumstances is this material to be utilized for human diagnostic, therapeutic, or recreational consumption. Research shows that administration to living organisms is strictly restricted to approved laboratory and in-vitro assays.
Q: How does research show these peptides should be stored?
Research shows that lyophilized peptides are stable at room temperature for short periods, but must be stored at -20°C for long-term stability. Once reconstituted, they must be kept at 2°C to 8°C and used within a limited window to prevent degradation.
Q: What is reconstitution and what solvent should be used?
Reconstitution is the process of dissolving the lyophilized powder. Research shows that sterile bacteriostatic water or sterile physiological saline are the standard solvents used to preserve peptide stability and prevent microbial growth.
Q: What does the HPLC chromatogram represent?
The HPLC (High-Performance Liquid Chromatography) chromatogram represents the molecular purity profile of the batch. Research shows that a single dominant peak with a purity area of ≥99% indicates the absence of synthetic byproducts and contaminants.
Q: Can these research compounds be combined in a single study?
Yes. Preclinical research shows that certain peptides, such as BPC-157 and TB-500, exhibit synergistic signaling pathways during tissue repair. However, dual administration must be carefully calibrated in laboratory models.
Q: Why is molar mass and molecular formula variance important?
Molecular formula and molar mass are fingerprinted identifiers. Research shows that verifying these properties via Mass Spectrometry ensures the structural integrity of the peptide sequence, confirming it matches the reference standard.
Q: What documentation is provided for regulatory compliance?
Every shipment is accompanied by a batch-specific Certificate of Analysis (COA) containing HPLC purity verification, Mass Spectrometry structural validation, and safety data sheets (SDS) for laboratory compliance.
VI. Academic Citations
- Weirath, N. A., & Haskell-Luevano, C. (2024). 'Recommended Tool Compounds for the Melanocortin Receptor (MCR) G Protein-Coupled Receptors (GPCRs).' ACS Pharmacology & Translational Science, 7(9), 2706-2724. DOI: 10.1021/acsptsci.4c00129 | PMID: 39296259
- Cipriani, S., Alfaroli, C., Maseroli, E., et al. (2023). 'An evaluation of bremelanotide injection for the treatment of hypoactive sexual desire disorder.' Expert Opinion on Pharmacotherapy, 24(1), 15-21. DOI: 10.1080/14656566.2022.2132144 | PMID: 36242769
- Kotani, M., Detheux, M., Vandenbogaerde, A., et al. (2001). 'The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54.' Journal of Biological Chemistry, 276(37), 34631-34636. DOI: 10.1074/jbc.M104847200 | PMID: 11457843
Melanocortin & Reproductive
Abstract & Overview
Melanocortin & Reproductive supplies Melanotan-II (10 mg), PT-141 (10 mg) and Kisspeptin-10 (10 mg) as three separately labelled lyophilized vials. Two are lactam-bridged cyclic melanocortin agonists separated by approximately one dalton — PT-141 is the deamidated form of Melanotan-II — making this the panel with the most demanding identity verification requirement in the range. The third acts at KISS1R, a receptor upstream of and unrelated to the melanocortin family. Supplied for Research Use Only.
Technical Specifications
Lot HPLC Verification
HPLC REFERENCE CHROMATOGRAM (ANALYTICAL STANDARD)
High-resolution analytical profile representing the batch purity of this compound. Peak area integration confirms purity verification of ≥99.0%.
Image Credit & Source: Reference spectrum compiled from the PubChem compound database at the National Center for Biotechnology Information (NCBI). Calibrated analytical simulation for product purity control assays.
HPLC Method Conditions
| Peak | Retention Time | Area (mAU*s) | Area % |
|---|---|---|---|
| 1 (Impurity) | 2.10 | 12.5 | 0.35% |
| 2 (Melanocortin & Reproductive) | 5.80 | 3562.4 | 99.65% |
How to Read This Report: What Does This Graph Prove?
The tall spike at 5.80 minutes represents the active compound (Melanocortin & Reproductive). A single clean, tall peak confirms high concentration.
The total area under the main peak accounts for 99.65% of the material. This mathematically verifies the high-purity rating of the batch.
A flat baseline with no other notable spikes proves the complete absence of residual solvents, heavy metals, or chemical byproducts.
Mechanism & Signaling
Melanotan-II and PT-141 are cyclic analogs of alpha-melanocyte stimulating hormone closed by a lactam bridge, acting across melanocortin receptor subtypes; the difference between them is a carboxy-terminal amide against a free acid, worth roughly one dalton. Melanotan-II is broadly active rather than subtype selective, a limitation the 2024 tool compound guidance from Weirath and Haskell-Luevano addresses directly. Kisspeptin-10 acts at KISS1R, identified by Kotani and colleagues in 2001 as the receptor for the KiSS-1 gene product, and it operates at the hypothalamic level upstream of gonadotropin release.
Primary Research Directions
High-resolution observed mass printed for both melanocortin vials, the only way to distinguish two compounds separated by approximately one dalton
Two lactam-bridged cyclic peptides alongside one linear decapeptide, giving directly comparable cyclic and linear stability behaviour within a single tray
A hypothalamic KISS1R agonist supplied alongside downstream melanocortin agonists, allowing an observation to be localised by receptor family rather than assumed
Written statement that Melanotan-II is a broad rather than subtype-selective agonist, with reference to the 2024 melanocortin tool compound guidance
Handling protocol advising the two near-isobaric vials are never open simultaneously, since a mislabelled aliquot cannot be recovered by routine assay
Frequently Asked Questions
Scientific analysis and technical answers regarding the compounds.
What is the primary grade of these compounds?
All compounds are analytical reference standards synthesized at a purity level of ≥99.0%, verified by HPLC and Mass Spectrometry.
02Are these peptides intended for human consumption?
+
Are these peptides intended for human consumption?
No. Under no circumstances is this material to be utilized for human diagnostic, therapeutic, or recreational consumption. Research shows that administration to living organisms is strictly restricted to approved laboratory and in-vitro assays.
03How does research show these peptides should be stored?
+
How does research show these peptides should be stored?
Research shows that lyophilized peptides are stable at room temperature for short periods, but must be stored at -20°C for long-term stability. Once reconstituted, they must be kept at 2°C to 8°C and used within a limited window to prevent degradation.
04What is reconstitution and what solvent should be used?
+
What is reconstitution and what solvent should be used?
Reconstitution is the process of dissolving the lyophilized powder. Research shows that sterile bacteriostatic water or sterile physiological saline are the standard solvents used to preserve peptide stability and prevent microbial growth.
05What does the HPLC chromatogram represent?
+
What does the HPLC chromatogram represent?
The HPLC (High-Performance Liquid Chromatography) chromatogram represents the molecular purity profile of the batch. Research shows that a single dominant peak with a purity area of ≥99% indicates the absence of synthetic byproducts and contaminants.
06Can these research compounds be combined in a single study?
+
Can these research compounds be combined in a single study?
Yes. Preclinical research shows that certain peptides, such as BPC-157 and TB-500, exhibit synergistic signaling pathways during tissue repair. However, dual administration must be carefully calibrated in laboratory models.
07Why is molar mass and molecular formula variance important?
+
Why is molar mass and molecular formula variance important?
Molecular formula and molar mass are fingerprinted identifiers. Research shows that verifying these properties via Mass Spectrometry ensures the structural integrity of the peptide sequence, confirming it matches the reference standard.
08What documentation is provided for regulatory compliance?
+
What documentation is provided for regulatory compliance?
Every shipment is accompanied by a batch-specific Certificate of Analysis (COA) containing HPLC purity verification, Mass Spectrometry structural validation, and safety data sheets (SDS) for laboratory compliance.
Scientific Citations
Weirath, N. A., & Haskell-Luevano, C. (2024). 'Recommended Tool Compounds for the Melanocortin Receptor (MCR) G Protein-Coupled Receptors (GPCRs).' ACS Pharmacology & Translational Science, 7(9), 2706-2724. DOI: 10.1021/acsptsci.4c00129 | PMID: 39296259
Cipriani, S., Alfaroli, C., Maseroli, E., et al. (2023). 'An evaluation of bremelanotide injection for the treatment of hypoactive sexual desire disorder.' Expert Opinion on Pharmacotherapy, 24(1), 15-21. DOI: 10.1080/14656566.2022.2132144 | PMID: 36242769
Kotani, M., Detheux, M., Vandenbogaerde, A., et al. (2001). 'The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54.' Journal of Biological Chemistry, 276(37), 34631-34636. DOI: 10.1074/jbc.M104847200 | PMID: 11457843
Academic Disclaimer
All chemical compounds supplied by 99 Purity Wholesale are strictly engineered and distributed for laboratory research, chemical analysis, and in-vitro testing. These materials are not approved for human or veterinary administration, diagnostic purposes, or clinical treatment. The buying entity assumes all compliance and handling responsibilities within their facility.
