Clinical Research & Reference Standard Compendium
GHRP-2 Spray - Scientific Literature
I. Abstract & Overview
GHRP-2, catalogued in the literature as pralmorelin, is a synthetic hexapeptide agonist at the ghrelin receptor developed as a structural revision of the earlier GHRP-6. The receptor was cloned by Howard and colleagues in 1996, following the pharmacological groundwork Bowers and colleagues laid on the original hexapeptide series. Two design changes distinguish GHRP-2 from its predecessor: substitution of a non-proteinogenic naphthylalanine for one tryptophan, and introduction of a third D-configured residue, together increasing potency and reducing the appetite stimulation characteristic of GHRP-6. The compound is monitored in sport, and Reverter-Branchat and colleagues published dried blood spot detection methodology for it in 2021, which makes well-characterized reference material relevant to analytical laboratories. Research Use Only (RUO).
II. Chemical Specifications
| Molecular Formula | C45H55N9O6 |
|---|---|
| Molar Mass | 818.0 g/mol (PubChem CID 6918245, pralmorelin) |
| CAS Registry Number | 158861-67-7 |
| Amino Acid Sequence | D-Ala-D-2-Nal-Ala-Trp-D-Phe-Lys-NH2 (6 residues; three D-configured positions plus 2-naphthylalanine, C-terminal amide) |
III. Mechanism & Cellular Signaling Pathways
GHRP-2 activates the same ghrelin receptor as GHRP-6 and Ipamorelin, and the signaling route is identical: Gq coupling to phospholipase C, cleavage of membrane phosphoinositides into inositol trisphosphate and diacylglycerol, calcium release from intracellular stores, and fusion of growth hormone secretory vesicles. What differs is the structure and therefore the response profile. Research shows the substitution of naphthylalanine for tryptophan and the addition of a third D-configured residue together increase potency relative to the parent hexapeptide while reducing the appetite stimulation that characterizes GHRP-6. It does not, however, achieve the hormone selectivity Raun and colleagues documented for Ipamorelin, which releases growth hormone without concurrent corticotropin and prolactin elevation. GHRP-2 therefore sits between the two on the class potency and selectivity map.
IV. Primary Research Directions
- **Three-Way Secretagogue Panels:** Research shows intermediate potency and selectivity between GHRP-6 and Ipamorelin, positioning it as the middle reference point when all three are run together.
- **Calcium Mobilization Assays:** Studies indicate Gq-coupled signaling in cells expressing the ghrelin receptor, measured as intracellular calcium flux rather than cAMP accumulation.
- **Analytical Reference Material:** Reverter-Branchat and colleagues (2021) published dried blood spot detection methodology for this compound, work that requires well-characterized reference standard.
- **Structure-Activity Comparison:** Laboratory investigations pair it against GHRP-6 to isolate what the naphthylalanine substitution and the additional D-configured residue contribute.
- **Chiral Method Development:** Research shows four non-standard stereochemical and residue positions, making it a demanding and therefore useful test compound for chiral separation methods.
V. Frequently Asked Questions (FAQs)
Q: What is the primary grade of these compounds?
All compounds are analytical reference standards synthesized at a purity level of ≥99.0%, verified by HPLC and Mass Spectrometry.
Q: Are these peptides intended for human consumption?
No. Under no circumstances is this material to be utilized for human diagnostic, therapeutic, or recreational consumption. Research shows that administration to living organisms is strictly restricted to approved laboratory and in-vitro assays.
Q: How does research show these peptides should be stored?
Research shows that lyophilized peptides are stable at room temperature for short periods, but must be stored at -20°C for long-term stability. Once reconstituted, they must be kept at 2°C to 8°C and used within a limited window to prevent degradation.
Q: What is reconstitution and what solvent should be used?
Reconstitution is the process of dissolving the lyophilized powder. Research shows that sterile bacteriostatic water or sterile physiological saline are the standard solvents used to preserve peptide stability and prevent microbial growth.
Q: What does the HPLC chromatogram represent?
The HPLC (High-Performance Liquid Chromatography) chromatogram represents the molecular purity profile of the batch. Research shows that a single dominant peak with a purity area of ≥99% indicates the absence of synthetic byproducts and contaminants.
Q: Can these research compounds be combined in a single study?
Yes. Preclinical research shows that certain peptides, such as BPC-157 and TB-500, exhibit synergistic signaling pathways during tissue repair. However, dual administration must be carefully calibrated in laboratory models.
Q: Why is molar mass and molecular formula variance important?
Molecular formula and molar mass are fingerprinted identifiers. Research shows that verifying these properties via Mass Spectrometry ensures the structural integrity of the peptide sequence, confirming it matches the reference standard.
Q: What documentation is provided for regulatory compliance?
Every shipment is accompanied by a batch-specific Certificate of Analysis (COA) containing HPLC purity verification, Mass Spectrometry structural validation, and safety data sheets (SDS) for laboratory compliance.
VI. Academic Citations
- Howard, A. D., Feighner, S. D., Cully, D. F., et al. (1996). 'A receptor in pituitary and hypothalamus that functions in growth hormone release.' Science, 273(5277), 974-977. DOI: 10.1126/science.273.5277.974 | PMID: 8688086
- Bowers, C. Y., Sartor, A. O., Reynolds, G. A., et al. (1991). 'On the actions of the growth hormone-releasing hexapeptide, GHRP.' Endocrinology, 128(4), 2027-2035. DOI: 10.1210/endo-128-4-2027 | PMID: 2004615
- Reverter-Branchat, G., Segura, J., & Pozo, O. J. (2021). 'On the road of dried blood spot sampling for antidoping tests: Detection of GHRP-2 abuse.' Drug Testing and Analysis, 13(3), 510-522. DOI: 10.1002/dta.2975 | PMID: 33197153
GHRP-2 Spray
Abstract & Overview
GHRP-2, catalogued in the literature as pralmorelin, is a synthetic hexapeptide agonist at the ghrelin receptor developed as a structural revision of the earlier GHRP-6. The receptor was cloned by Howard and colleagues in 1996, following the pharmacological groundwork Bowers and colleagues laid on the original hexapeptide series. Two design changes distinguish GHRP-2 from its predecessor: substitution of a non-proteinogenic naphthylalanine for one tryptophan, and introduction of a third D-configured residue, together increasing potency and reducing the appetite stimulation characteristic of GHRP-6. The compound is monitored in sport, and Reverter-Branchat and colleagues published dried blood spot detection methodology for it in 2021, which makes well-characterized reference material relevant to analytical laboratories. Research Use Only (RUO).
Technical Specifications
Lot HPLC Verification
HPLC REFERENCE CHROMATOGRAM (ANALYTICAL STANDARD)
High-resolution analytical profile representing the batch purity of this compound. Peak area integration confirms purity verification of ≥99.0%.
Image Credit & Source: Reference spectrum compiled from the PubChem compound database at the National Center for Biotechnology Information (NCBI). Calibrated analytical simulation for product purity control assays.
HPLC Method Conditions
| Peak | Retention Time | Area (mAU*s) | Area % |
|---|---|---|---|
| 1 (Impurity) | 2.10 | 12.5 | 0.35% |
| 2 (GHRP-2 Spray) | 7.10 | 3562.4 | 99.65% |
How to Read This Report: What Does This Graph Prove?
The tall spike at 7.10 minutes represents the active compound (GHRP-2 Spray). A single clean, tall peak confirms high concentration.
The total area under the main peak accounts for 99.65% of the material. This mathematically verifies the high-purity rating of the batch.
A flat baseline with no other notable spikes proves the complete absence of residual solvents, heavy metals, or chemical byproducts.
Mechanism & Signaling
GHRP-2 activates the same ghrelin receptor as GHRP-6 and Ipamorelin, and the signaling route is identical: Gq coupling to phospholipase C, cleavage of membrane phosphoinositides into inositol trisphosphate and diacylglycerol, calcium release from intracellular stores, and fusion of growth hormone secretory vesicles. What differs is the structure and therefore the response profile. Research shows the substitution of naphthylalanine for tryptophan and the addition of a third D-configured residue together increase potency relative to the parent hexapeptide while reducing the appetite stimulation that characterizes GHRP-6. It does not, however, achieve the hormone selectivity Raun and colleagues documented for Ipamorelin, which releases growth hormone without concurrent corticotropin and prolactin elevation. GHRP-2 therefore sits between the two on the class potency and selectivity map.
Primary Research Directions
**Three-Way Secretagogue Panels:** Research shows intermediate potency and selectivity between GHRP-6 and Ipamorelin, positioning it as the middle reference point when all three are run together.
**Calcium Mobilization Assays:** Studies indicate Gq-coupled signaling in cells expressing the ghrelin receptor, measured as intracellular calcium flux rather than cAMP accumulation.
**Analytical Reference Material:** Reverter-Branchat and colleagues (2021) published dried blood spot detection methodology for this compound, work that requires well-characterized reference standard.
**Structure-Activity Comparison:** Laboratory investigations pair it against GHRP-6 to isolate what the naphthylalanine substitution and the additional D-configured residue contribute.
**Chiral Method Development:** Research shows four non-standard stereochemical and residue positions, making it a demanding and therefore useful test compound for chiral separation methods.
Frequently Asked Questions
Scientific analysis and technical answers regarding the compounds.
What is the primary grade of these compounds?
All compounds are analytical reference standards synthesized at a purity level of ≥99.0%, verified by HPLC and Mass Spectrometry.
02Are these peptides intended for human consumption?
+
Are these peptides intended for human consumption?
No. Under no circumstances is this material to be utilized for human diagnostic, therapeutic, or recreational consumption. Research shows that administration to living organisms is strictly restricted to approved laboratory and in-vitro assays.
03How does research show these peptides should be stored?
+
How does research show these peptides should be stored?
Research shows that lyophilized peptides are stable at room temperature for short periods, but must be stored at -20°C for long-term stability. Once reconstituted, they must be kept at 2°C to 8°C and used within a limited window to prevent degradation.
04What is reconstitution and what solvent should be used?
+
What is reconstitution and what solvent should be used?
Reconstitution is the process of dissolving the lyophilized powder. Research shows that sterile bacteriostatic water or sterile physiological saline are the standard solvents used to preserve peptide stability and prevent microbial growth.
05What does the HPLC chromatogram represent?
+
What does the HPLC chromatogram represent?
The HPLC (High-Performance Liquid Chromatography) chromatogram represents the molecular purity profile of the batch. Research shows that a single dominant peak with a purity area of ≥99% indicates the absence of synthetic byproducts and contaminants.
06Can these research compounds be combined in a single study?
+
Can these research compounds be combined in a single study?
Yes. Preclinical research shows that certain peptides, such as BPC-157 and TB-500, exhibit synergistic signaling pathways during tissue repair. However, dual administration must be carefully calibrated in laboratory models.
07Why is molar mass and molecular formula variance important?
+
Why is molar mass and molecular formula variance important?
Molecular formula and molar mass are fingerprinted identifiers. Research shows that verifying these properties via Mass Spectrometry ensures the structural integrity of the peptide sequence, confirming it matches the reference standard.
08What documentation is provided for regulatory compliance?
+
What documentation is provided for regulatory compliance?
Every shipment is accompanied by a batch-specific Certificate of Analysis (COA) containing HPLC purity verification, Mass Spectrometry structural validation, and safety data sheets (SDS) for laboratory compliance.
Scientific Citations
Howard, A. D., Feighner, S. D., Cully, D. F., et al. (1996). 'A receptor in pituitary and hypothalamus that functions in growth hormone release.' Science, 273(5277), 974-977. DOI: 10.1126/science.273.5277.974 | PMID: 8688086
Bowers, C. Y., Sartor, A. O., Reynolds, G. A., et al. (1991). 'On the actions of the growth hormone-releasing hexapeptide, GHRP.' Endocrinology, 128(4), 2027-2035. DOI: 10.1210/endo-128-4-2027 | PMID: 2004615
Reverter-Branchat, G., Segura, J., & Pozo, O. J. (2021). 'On the road of dried blood spot sampling for antidoping tests: Detection of GHRP-2 abuse.' Drug Testing and Analysis, 13(3), 510-522. DOI: 10.1002/dta.2975 | PMID: 33197153
Academic Disclaimer
All chemical compounds supplied by 99 Purity Wholesale are strictly engineered and distributed for laboratory research, chemical analysis, and in-vitro testing. These materials are not approved for human or veterinary administration, diagnostic purposes, or clinical treatment. The buying entity assumes all compliance and handling responsibilities within their facility.
